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2 edition of Studies of the HIV-1 vif gene found in the catalog.

Studies of the HIV-1 vif gene

Mark Boyce

Studies of the HIV-1 vif gene

by Mark Boyce

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  • 7 Currently reading

Published by typescript in [s.l.] .
Written in English


Edition Notes

Thesis (Ph.D.) - University of Warwick, 1998.

StatementMark Boyce.
The Physical Object
Paginationxx, 259p.
Number of Pages259
ID Numbers
Open LibraryOL18441272M

HIV-1 Vif is expressed in E. coli and refolded from inclusion bodies (Supplementary Fig. S3A). Refolded HIV-1 Vif was soluble and stable at low concentrations (Vif revealed a β-character protein (Supplementary Fig. S3B), consistent with previous studies A3Fc-CD2 bound to. HIV-1, Molecular Biology and Pathogenesis Viral Mechanisms. Advances in Pharmacology, Volume xusyh HIV-1 Molecular Biology and Pathogenesis Viral Mechanisms.

To study the effect of gene overlapping on PID more explicitly, we decided to split the data into eight HIV-1 genes (tat, rev, gag, pol, vif, vpr, env, and vpu).   PCR products were inserted into two versions of the NL HIV-1 molecular clone, pNL vifA and pNL vifB, differing at only six synonymous nucleotides in the HIV-1 vif gene, using a previously described yeast-based recombination system (Figure S1 online).

Figure ce alignment and genome organization around the vif gene of HIV-1 proviral clones used in this study. Proviral clones of HIV-1 subtype B (NL, HIV-1 NL; GenBank: AF) (Adachi et al., ) and subtype C (Indie, HIV-1 IndieC1; GenBank: AB) (Mochizuki et al., ) were used in this in the nucleotide and amino acid sequences of Indie show the .   The viral infectivity factor, Vif, of human immunodeficiency virus type 1, HIV-1, has long been shown to promote viral replication in vivo and to serve a critical function for productive infection of non-permissive cells, like peripheral blood mononuclear cells (PBMC). Vif functions to counteract an anti-retroviral cellular factor in non-permissive cells named APOBEC3G.


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Studies of the HIV-1 vif gene by Mark Boyce Download PDF EPUB FB2

The viral infectivity factor (Vif) of HIV type-1 (HIV-1) is essential for efficient viral replication, yet was, until recently, enigmatic. This resulted from the complexity and cellular specificity of its function and the correspondingly complex systems that are required for its by: To identify the current prevalent subtypes and to study the genetic variation of HIV-1 strains in men who have sex with men (MSM) residing in Heilongjiang province, China.

We analyzed the characteristics of the nucleotide sequences and the corresponding deduced protein of Vif of HIV-1 strains isolated from 17 drug-naive HIVseropositive by: 1.

In this study, we use a human hematopoietic stem cell-transplanted humanized mouse (NOG-hCD34 mouse) model and demonstrate the predominant accumulation of G-to-A mutations in vif-proficient HIV-1 provirus displaying characteristics of APOBEC3-mediated mutagenesis.

In this study, the genetic characterization of HIV-1 strains prevalent in the MSM in Shijiazhuang, China, was analyzed basing on the HIV-1 full-length gag, pol, and partial env gene. 21 drug.

HIV-1 Studies of the HIV-1 vif gene book is a 23KD viral accessory protein that is required for production of infectious virus in a cell type-specific manner [1, 2].Viruses lacking a functional vif gene are severely restricted in their ability to replicate in non-permissive cell types when compared to wild type viruses.

Non-permissive cell types include primary T cells and macrophages as well as some T cell lines (e.g. H9 Cited by:   Vif is a basic M r ∼23 phosphoprotein and is required for HIV-1 replication in cells that are termed nonpermissive, which include lymphocytes and macrophages and some leukemic T-cell lines 3, 4, 5, contrast, Vif is irrelevant for viral replication in cells that are termed permissive, which include other leukemic T-cell lines and commonly used non-hematopoietic cell lines, such as.

Studies have shown that Vif binds to HIV-1 RNA, however, the mechanisms by which Δvif HIV-1 is generated remain uncl 11, A major clue to the solution of this mystery came in by two independent groups, Madani and Kabat and Simon et al.

In somatic cell fusion experiments, heterokaryons formed by the fusion of permissive and non. The viral infectivity factor gene vif of human immunodeficiency virus type 1 (HIV-1) has been shown to enhance the cellfree infectivity of HIV-1 virus particles.

Previous studies have demonstrated that vif increases viral infectivity at the time of virus production, most likely by affecting viral protein processing, virus assembly, or virus maturation. Deaminase-Independent Antiviral Mechanisms.

Although A3G-mediated deamination was initially proposed to be the sole mechanism of the antiviral activity against vif-deficient HIV-1, subsequent studies have demonstrated that other mechanisms are also involved in the inhibition of viral addition, the enzymatic activity of A3F is not absolutely required for its inhibitory effect on.

Hence, the HIV-1 mutation rate in vivo, the contribution of the HIV-1 RT, and host A3 proteins to this rate, as well as its relevance to disease progression, remain to be elucidated.

In this study, we estimate the mutation rate of HIV-1 using sequences derived from both intracellular viral DNA and plasma viral RNA. These findings suggest that each of the three regions mutated in vif is critical for function. Our observations are consistent with studies showing marked attenuation of HIV-1 vif mutants in certain cell types.

We conclude that the vif gene is a critical determinant of FIV-pF34 replication and infectivity in CrFK and G cells. Study design: We have employed the yeast 2-hybrid system to identify cellular proteins which interact with HIV-1 Vif.

Sixteen clones were isolated from a high stringency yeasthybrid screen of a human leucocyte cDNA library with Vif derived from the T-cell tropic HIV-1 strain NL Acquired immunodeficiency syndrome is a pandemic disease due to increased variability in causative agent in global distribution; it is attributed to various complications in developing the vaccine.

Phylogenetic analysis of the vif gene indicated that subtype B was predominant in ∼77% (/) of the patients, followed by HIV-1 F ∼18% (56/), and subtype C ∼4% (12/); five samples were BF recombinants (∼1% of patients), and one was an AG recombinant.

Thus, nonpermissive H9 cells were exposed to virus produced by HeLa cells transfected with wild-type HIV-1 (pNL) or a variant in which a portion of the vif gene had been deleted (Δvif). The virions produced by H9 cells 24 to 48 hours after infection were recovered. Author: Boyce M. Search worldwide, life-sciences literature Search.

Advanced Search. E.g. "breast cancer" HER2 Smith J. To study the biological activity of HIV-1 Vif gene products from different HIV-infected individuals, we initially selected 14 samples from the Mother to Child Transmission cohort in the Pumwani area of Nairobi, Kenya, based on their survival period and related epidemiology data and tried to amplify and clone vif genes into a pCR4Blunt-TOPO vector for further studies.

Nef, an early gene of HIV, is the first viral protein to accumulate to detectable levels in a cell following HIV-1 infection. Its name is a consequence of early reports claiming that Nef down-regulated transcriptional activity of the HIV-1 LTR.

It is no longer believed, however, that Nef has a direct effect on HIV gene expression. Title: HIV-1 Vif: HIVs Weapon Against the Cellular Defense Factor APOBEC3G VOLUME: 3 ISSUE: 4 Author(s):Melanie Kremer and Barbara S.

Schnierle Affiliation:Department of Virology, Paul-Ehrlich-Institut, Paul-Ehrlich StrasseLangen, Germany. Keywords:human immunodeficiency virus, vif, apobec g, innate immunity Abstract: The human immunodeficiency virus type 1, HIV-1, has long. Viral infectivity factor, or Vif, is an accessory protein found in HIV and other role is to disrupt the antiviral activity of the human enzyme APOBEC (specifically APOBEC3G, "A3G" in short) by targeting it for ubiquitination and cellular degradation.

APOBEC is a cytidine deaminase enzyme that mutates viral nucleic acids. Vif is a kilodalton protein that is essential for. In other studies, a macaque-tropic HIV-1 derivative with the SIV vif gene and a short 21 base pair segment corresponding to the HIV-1 cyclophilin A binding loop from SIV was constructed (NL-DT5R; Kamada et al., ; Igarashi et al., ).

The virus showed increased infectivity in both CM and PTM T. To study the immunogenicity of the HIV-1 vif DNA vaccine in inducing the humoral and cellular immune responses and the immunoadjuvant effect of LIGHT, which is a member of TNF superfamily and can stimulate the proliferation of naïve T cells as a co-stimulatory molecule, DNA vaccine plasmid pcDNA-Vif was constructed by inserting HIV-1 vif gene.The human immunodeficiency virus type 1 (HIV-1) genome (~9 kb RNA) is flanked by two long terminal repeats (LTR) promoter regions with nine open reading frames, which encode Gag, Pol and Env polyproteins, four accessory proteins (Vpu, Vif, Vpr, Nef) and two regulatory proteins (Rev, Tat).

In this study, we carried out a genome-wide and functional analysis of the HIV-1 genome in fission yeast.